Annotation Comparison

This document was produced to allow users to evaluate the annotations produced by the RAST server. We ran five genomes through the RAST server and the KEGG Automatic Annotation Server at We then collected current annotations of the protein encoding genes from a number of sources. We gathered all of the results and built five tables -- one for each genome. We suggest that you simply scroll through these tables and examine the differences. These tables were put together in mid-November, 2007. We believe both the RAST server and the annotations from other sources will continue to improve rapidly. If you really need a detailed analysis that is current, we suggest you run a genome that you are familiar with through the RAST server and evaluate the output.

RAST calls were obtained by submitting the DNA sequence of the genome (GenBank format obtained from RefSeq) to the RAST server at

Annotation Clearing House was queried using the GenBank Ids of the genes (at

KEGG calls were obtained by submitting the protein sequences (obtained from RefSeq) to the KEGG Automatic Annotation Server at

The tables show a comparison of the RAST annotations to the annotations of the genes in the ACH as well as protein annotations obtained from the KAAS. The KAAS output provides you with a KO (KEGG Orthology) number that we have attempted to connect to a KO assignment. In our table, the RAST, ACH and KO assignments link back to the appropriate websites.

Comparison for:

GenomeSlow inet connectionFast inet connection
Bacillus subtilis subsp. subtilis str. 168100 genes/pageall on one page
Escherichia coli K12100 genes/pageall on one page
Staphylococcus aureus subsp. aureus COL100 genes/pageall on one page
Synechocystis sp. PCC 6803100 genes/pageall on one page
Vibrio cholera cholerae O1 biovar eltor str. N16961100 genes/pageall on one page